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goat polyclonal antibody against mouse il 36α  (R&D Systems)


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    R&D Systems goat polyclonal antibody against mouse il 36α
    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d <t>Interleukin-36α</t> (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Goat Polyclonal Antibody Against Mouse Il 36α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti+il+36%CE%B1/Mouse+IL-36+alpha%2FIL-1F6+Antibody/pmc12929778-330-8-23
    Average 93 stars, based on 21 article reviews
    goat polyclonal antibody against mouse il 36α - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Bone mineral loss damages renal tubules in mice"

    Article Title: Bone mineral loss damages renal tubules in mice

    Journal: Communications Biology

    doi: 10.1038/s42003-026-09603-0

    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Figure Legend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Techniques Used: Quantitative RT-PCR, Comparison, Clinical Proteomics, Immunohistochemistry, Injection

    a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).
    Figure Legend Snippet: a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Techniques Used: Clinical Proteomics, Comparison

    Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.
    Figure Legend Snippet: Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Techniques Used: Clinical Proteomics

    Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.
    Figure Legend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Techniques Used: Quantitative RT-PCR, Comparison, Staining, Immunohistochemistry

    Related Articles

    Immunohistochemistry:

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Staining:

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Control:

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Article Title: IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis
    Article Snippet: Statistical significance between healthy, PV and GPP samples was determined using a Kruskal-Wallace test with Dunn’s multiple comparison test in GraphPad Prism v6. .. Immunohistochemistry was performed on formalin fixed and paraffin embedded human skin biopsies sectioned at 5 μm, and then immunohistochemically stained after antigen retrieval using polyclonal goat anti-IL-36α (1μg/ml, AF1078, R&D Systems), IL-36γ (2.5μg/ml, rat, Y-12, Santa Cruz Biotech), polyclonal goat anti-CXCL8 (5μg/ml, AF2008, R&D Systems), polyclonal rabbit anti-cathepsin G (5μg/ml, NBP233498, Novus Biologicals), neutrophil elastase (1μg/ml, mouse, NP57, Dako), proteinase 3 (1μg/ml, EPR6277, LSBio), and matched isotype control antibodies. ..

    Immunohistochemical staining:

    Article Title: Increased neutrophil-derived IL-17A identified in generalized pustular psoriasis.
    Article Snippet: Generalized pustular psoriasis (GPP), a severe form of psoriasis characterized by sudden extensive development of sterile pustules, is more common in Asians than Caucasians, accounting for approximately 1% of all psoriasis cases.. As a systemic inflammatory disorder involving multiple organs, GPP is often accompanied by high fever, leukocytosis and hypoproteinaemia, or even combined with multiple arthritis, neutrophil cholangitisinduced cholestasis, or lifethreatening acute respiratory distress syndrome.1 GPP is currently divided into PSO+GPP subtypes and PSO−GPP subtypes, which are distinguished by the history of psoriasis vulgaris (PV) before firstonset of GPP.. GPP with an interleukin (IL)36RN mutation is characterized by the deficiency of interleukin 36 receptor antagonist (DITRA), accounting for 10%– 60.5% of GPP patients.2,3 Received: 26 September 2023 | Revised: 18 January 2024 | Accepted: 21 January 2024 DOI: 10.1111/exd.15026

    Expressing:

    Article Title: Increased neutrophil-derived IL-17A identified in generalized pustular psoriasis.
    Article Snippet: Generalized pustular psoriasis (GPP), a severe form of psoriasis characterized by sudden extensive development of sterile pustules, is more common in Asians than Caucasians, accounting for approximately 1% of all psoriasis cases.. As a systemic inflammatory disorder involving multiple organs, GPP is often accompanied by high fever, leukocytosis and hypoproteinaemia, or even combined with multiple arthritis, neutrophil cholangitisinduced cholestasis, or lifethreatening acute respiratory distress syndrome.1 GPP is currently divided into PSO+GPP subtypes and PSO−GPP subtypes, which are distinguished by the history of psoriasis vulgaris (PV) before firstonset of GPP.. GPP with an interleukin (IL)36RN mutation is characterized by the deficiency of interleukin 36 receptor antagonist (DITRA), accounting for 10%– 60.5% of GPP patients.2,3 Received: 26 September 2023 | Revised: 18 January 2024 | Accepted: 21 January 2024 DOI: 10.1111/exd.15026



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    goat polyclonal antibody against mouse il 36α  (R&D Systems)
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    R&D Systems goat polyclonal antibody against mouse il 36α
    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d <t>Interleukin-36α</t> (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Goat Polyclonal Antibody Against Mouse Il 36α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d <t>Interleukin-36α</t> (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
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    Image Search Results


    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Quantitative RT-PCR, Comparison, Clinical Proteomics, Immunohistochemistry, Injection

    a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Clinical Proteomics, Comparison

    Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Clinical Proteomics

    Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Quantitative RT-PCR, Comparison, Staining, Immunohistochemistry